ANALYSIS OF POLYMORPHISM OF THE GENES OF THE PHASE II OF XENOBIOTICS BIOTRANSFORMATION (GSTM1, GSTT1) IN THE SHORIANS AND ALIEN POPULATION OF THE KEMEROVO REGION: THE PROBLEM OF THE DIFFERENCES IN MORTALITY RATE FROM MALIGNANT NEOPLASMS
Main Article Content
Abstract
Subject. Polymorphism of the genes of xenobiotics biotransformation GSTM1, GSTT1 (probable markers of cancer risk) in the Shorians and Europeoid population of the Kemerovo Region; mortality rates from malignant neoplasms (MNP) in the Shorians and the entire population in Novokuznetsk for the period from 1970 to 1999.
Objective. To study the peculiarities of the distribution of the gene polymorphism of xenobiotics biotransformation (GSTM1, GSTT1) in the indigenous and alien population of the Kemerovo Region. To analyze ethno-genetic aspects of mortality rate from malignant neoplasms (on the example of Novokuznetsk).
Materials and methods. The adult indigenous and alien population of Gornaya Shoria and Novokuznetsk of the Kemerovo Region was examined. DNA samples were selected from the venous blood by the phenol-chloroform extraction method. The deletion polymorphism of the GSTM1 and GSTT1 genes was studied by PCR method in real-time using LLC «SibDNA» reagent kit. We used data on mortality rates from MNP among the Shorians obtained from the archives of the recording of the acts of civil status in Novokuznetsk.
Main results. The indigenous Shorians are dominated by the frequencies of the non-mutant variants of the genes of xenobiotics biotransformation: GSTM1 (+) is 0.768, GSTT1 (+) is 0.743; the frequency of «null» functionally weakened genotypes and their combinations compared with the Europeoid population is reduced. In the structure of the classes of death causes in the Shorians in the 90th years neoplasms are at the fourth ranked place (6.3 %), for the entire population of Novokuznetsk they are at the third one (11.7 %).
Conclusions. The reduced frequency of the deletion variants of the genes of xenobiotics biotransformation (GSTM1, GSTT1) in the Shorians allows predicting the risk of disease incidence and mortality rates from oncologic pathology at the population level. Under the same ecological conditions of the urban environment mortality rates from MNP among the Shorians occupy a lower rank in the structure of the main classes of death causes in the comparison with the population of Novokuznetsk.Keywords
Article Details
Information about financing and conflict of interests
The authors declare that they have no apparent or potential conflicts of interest related to the publication of this article.
This work is licensed under a Creative Commons Attribution 4.0 License.
How to Cite
References
Genetic passport is the basis of individual and predictive medicine. Baranov VS, editor. St. Petersburg: N-L Publ., 2009. 528 p. Russian (Генетический паспорт – основа индивидуальной и предиктивной медицины /под ред. В.С. Баранова. СПб.: Изд-во Н-л, 2009. 528 с.)
Gulyaeva LF, Vavilin VA, Lyakhovich VV. Enzymes of xenobiotics biotransformation in chemical carcinogenesis. Ecology. A series of analytical reviews of world literature. 2000; (57): 1-85. Russian (Гуляева Л.Ф., Вавилин В.А., Ляхович В.В. Ферменты биотрансформации ксенобиотиков в химическом канцерогенезе //Экология. Серия аналитических обзоров мировой литературы. 2000. №. 57. С. 1-85)
Ivanov VP, Polonikov AV, Khoroshaya IV, Solodilova MA, Belugin DA. Polymorphism of the genes of the enzymes of the system of xenobiotics biotransformation in humans and its importance for ecological genetics. In: The materials of the International scientific conference «Ecology, Environment and Health of the Population of the Central Chernozem Region». Kursk, 2005. P. 211-213. Russian (Иванов В.П., Полоников А.В., Хорошая И.В., Солодилова М.А., Белугин Д.А. Полиморфизм генов ферментов системы биотрансформации ксенобиотиков у человека и его значение для экологической генетики //Матер. Междунар. науч. конф. «Экология, окружающая среда и здоровье населения Центрального Черноземья». Курск, 2005. С. 211-213)
Spitsyn VA. Ecological human genetics. Moscow: Science Publ., 2008. 503 p. Russian (Спицын В.А. Экологическая генетика человека. М.: Наука, 2008. 503 с.)
World Health Organization. Information bulletin. Cancer. February 2017. Available at: http://www.who.int/mediacentre/factsheets/fs297/ru/ (accessed 01.09.2017). Russian (ВОЗ. Информационный бюллетень. Рак. Февраль 2017 г. Режим доступа: http://www.who.int/mediacentre/factsheets/fs297/ru/ (дата обращения: 01.09.2017))
Larin SA, Brailovsky VV, Mun SA, Eremina NA, Glushkov AN. A new approach to assessing oncologic disease incidence in the region on the example of Kuzbass. Medicine in Kuzbass. 2002; (1): 41-44. Russian (Ларин С.А., Браиловский В.В., Мун С.А., Еремина Н.А., Глушков А.Н. Новый подход к оценке онкологической заболеваемости в регионе на примере Кузбасса //Медицина в Кузбассе. 2002. № 1. С. 41-44)
Kolyado VB. Losses of the population health from exposure to radioactive fallout during nuclear tests (retrospective medical and demographic diagnosis and evaluation). Barnaul, 1998. 234 p. Russian (Колядо В.Б. Потери здоровья населения от облучения радиоактивными осадками при ядерных испытаниях (ретроспективная медико-демографическая диагностика и оценка). Барнаул, 1998. 234 с.)
Malignant neoplasms in Russia in 2015 (morbidity and mortality). Caprin AD, Starinsky VV, Petrova GV, editors. Moscow, 2017. 250 p. Russian (Злокачественные новообразования в России в 2015 году (заболеваемость и смертность) /под ред. А.Д. Каприна, В.В. Старинского, Г.В. Петровой. М., 2017. 250 с.)
Chechenin GI. Habitat, health of the population of Novokuznetsk in 2006-2007. Novokuznetsk, 2008. 375 p. Russian (Чеченин Г.И. Среда обитания, состояние здоровья населения г. Новокузнецка в 2006-2007 гг. Новокузнецк, 2008. 375 с.)
Pisareva LF, Boyarkina AP, Hubert EA, Pyzhova IB, Kovaleva EV, Nedavnyaya IO et al. Epidemiology of malignant neoplasms in the indigenous and alien populations of the region of Siberia and the Far East. In: Socio-clinical problems of oncology: the materials of the scientific and practical conference. Kemerovo, 2001. p. 9-10. Russian (Писарева Л.Ф., Бояркина А.П., Губерт Э.А., Пыжова И.Б., Ковалева Е.В., Недавняя И.О. и др. Эпидемиология злокачественных новообразований у коренного и пришлого населения региона Сибири и Дальнего Востока //Социально-клинические проблемы онкологии: матер. науч.-практ. конф. Кемерово, 2001. С. 9-10)
Troisi R, Ganmaa D, dos Santos Silva I, Davaalkham D, Rosenberg PS, Rich-Edwards J et al. The role of hormones in the differences in the incidence of breast cancer between Mongolia and the United Kingdom. PLoS One. 2014; 9(12): 1-13
Luzina FA, Doroshilova AV, Zakharenkov VV, Kolbasko AV, Gafarov NI. Analysis of the genetic and demographic structure of the indigenous peoples of Southern Siberia. Complex problems of cardiovascular diseases. 2014; (3): 60. Russian (Лузина Ф.А., Дорошилова А.В., Захаренков В.В., Колбаско А.В., Гафаров Н.И. Анализ генетико-демографической структуры коренных народов Южной Сибири //Комплексные проблемы сердечно-сосудистых заболеваний. 2014. № 3. С. 60)
Korchagina RP, Osipova LP, Vavilova NA, Ermolenko NA, Voronina EN, Filipenko ML. Polymorphism of genes for xenobiotic biotransformation, GSTM1, GSTT1, CYP2D6, candidate markers of cancer risk, in indigenous peoples and Russians in Nothern Siberia. Vavilovsky Journal of Genetics and Selection. 2011; 15 (3): 448-461. Russian (Корчагина Р.П., Осипова Л.П., Вавилова Н.А., Ермоленко Н.А., Воронина Е.Н., Филипенко М.Л. Полиморфизм генов биотрансформации ксенобиотиков GSTM1, GSTT1, CYP2D6, вероятных маркеров риска онкологических заболеваний, в популяциях коренных этносов и русских Северной Сибири //Вавиловский журнал генетики и селекции. 2011. Т. 15, № 3. С. 448-461)
Khudoley VV. Genes and enzymes of metabolic activation of xenobiotics in chemical carcinogenesis. Ecological genetics. 2003; 1(S): 30-35. Russian (Худолей В.В. Гены и ферменты метаболической активации ксенобиотиков в химическом канцерогенезе //Экологическая генетика. 2003. Т. 1, № S. С. 30-35)