"SKIN WINDOW" EXUDATE IL-1Β AND IL-18 IN PATIENTS WITH CHRONIC URTICARIA
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Abstract
Research subject. 26 patients at the age of 18 to 45 years with chronic urticaria were studied. The control group was composed of 25 healthy persons of 20 to 35 age.
Research purpose was to pick up a cell-free «skin window» exudate fraction and study its pro-inflammatory cytokine values in chronic urticaria.
Methods. The method of «skin window» chamber modified by Klimov V.V. et al. (patent № 1534395 and medical technology ФС № 2010.217) has been used to evaluate cytokine IL-1β and IL-18 values in the skin. The ELISA was exploited to assess cytokine concentrations in exudates, whereas they were previously standardized using the microbiuret approach in relation to the total protein value.
Results. At the «shock organ» level, a statistical validity regarding the control increase in pro-inflammatory cytokines IL-1β and IL-18 was revealed in chronic urticaria. It can be a hidden cause behind inflammasomes as multimolecular complexes in many inflammatory cells in the skin.
Conclusions. In chronic urticaria, the study of «skin window» exudate pro-inflammatory cytokines is important for such «shock organ» as the skin that enables the assessment of inflammatory activity and may correspond to inflammasome formation.
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References
Ilina N.I., Danilycheva I.V., Dorofeeva M.S. et al. Chronic urticaria in theory and practice. UCARE center experience committed to practitioners. Russian Allergy Journal. 2021; 18(1): 79-96. Russian (Ильина Н.И., Данилычева И.В., Дорофеева И.В. и др. Хроническая крапивница в теории и практике. Опыт UCARE-центров – практическим врачам //Российский аллергологический журнал. 2021. Т. 18, № 1. С. 79-96)
Pirozhkov SV, Litvitsky PF. Inflammasome diseases. Immunology. 2018; 39(2-3): 158-165. Russian (Пирожков С.В., Литвицкий П.Ф. Инфламмасомные болезни //Иммунология. 2018. Т. 39, № 2-3. С. 158-165)
Medical technology entitled «Method of the minimal skin inflammatory activity in atopic dermatitis remission stage /Klimov VV, Denisov AA, Firsova EK, Salikova TI, Zagreshenko DS. //ФС N 2010/217, date: 10.06.2010. Russian (Медицинская технология «Способ оценки минимальной воспалительной активности кожи при атопическом дерматите в стадии ремиссии» /Климов В.В., Денисов А.А., Фирсова Е.К., Саликова Т.И., Загрешенко Д.С. //ФС № 2010/217 от 10.06.2010)
Tang L, Zhou F. Inflammasomes in common immune-related skin diseases. Front Immunol. 2020; 11: 882. doi: 10.3389/fimmu.2020.00882
Lee JH, Cho DH, Park HJ. IL-18 and Cutaneous Inflammatory Diseases. International Journal of Molecular Sciences. 2015; 16(12): 29357-29369