ОЦЕНКА ИЗОЛИРОВАННОГО ВОЗДЕЙСТВИЯ ФИЗИЧЕСКИХ И ХИМИЧЕСКИХ ФАКТОРОВ НА ВАРИАБЕЛЬНОСТЬ БИОХИМИЧЕСКИХ ПОКАЗАТЕЛЕЙ КРОВИ В ХРОНИЧЕСКОМ ЭКСПЕРИМЕНТЕ НА ЛАБОРАТОРНЫХ ЖИВОТНЫХ

Abstract

The study of the features of biochemical changes in blood parameters in laboratory animals (6-month-old Wistar rats) that have been under periodic isolated exposure to physical and chemical factors for a long time under model conditions (180 -day chronic experiment) contributes to the diagnosis of the initial clinical manifestations of diseases associated with exposure to environmental factors affecting the development of accelerated cellular aging.
The purpose of this study is to evaluate the isolated effect of physical and chemical factors on the variability of biochemical blood parameters in a chronic experiment on laboratory animals.
Materials and methods. The experiment involved 120 Wistar laboratory rats divided into 4 groups of 30 individuals (15 females and 15 males) each, in which clinically important biochemical blood parameters were determined and evaluated over a 180-day period with an interval of 2 months, compared with the control group. The experiment was conducted in accordance with the rules adopted by the European Convention for the Protection of Animals Used for Experimental Scientific Purposes (Strasbourg, 1986), Guidelines for the Care of Laboratory Animals and their Use (Washington, 2011), after approval by the Ethics Commission of the Novosibirsk Research Institute of Hygiene of Rospotrebnadzor.
Results and their discussion. During the study, the limits of variability of biochemical blood parameters in Wistar laboratory rats were determined depending on the type and duration of exposure to physical (general vibration, noise) and chemical (4-component hydrocarbon aerosol) factors, in comparison with the control group. Based on the assessment of the results of biochemical blood parameters, markers of an increased risk of pathology of internal organs and premature aging in animals of the experimental groups were established, compared with the control group.
Conclusion. The results of a chronic experiment to study the isolated effects of physical and chemical factors (at the level of 1.5 MPC and MPC) on the biochemical parameters of Wistar rats indicate a change in the biochemical composition of blood on days 60-180, which increases the risk of stress-induced pathological conditions on day 180 of the experiment in animals of experimental groups, and may negatively affect the timing their lives.