ASSESSMENT OF THE MORPHOLOGICAL STATE OF TARGET ORGANS IN WISTAR RATS THAT WERE EXPOSED TO COMBINED VIBRATION AND NOISE IN A CHRONIC EXPERIMENT
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Abstract
The assessment of the morphological state of the target organs of Wistar rats exposed to combined general vibration and noise in a chronic experiment model helps to identify the initial pathological changes in the internal organs that trigger accelerated aging processes.
The aim of the study is to assess the morphological condition of the target organs of Wistar rats that were subjected to combined exposure to overall vibration and noise in the model conditions of a chronic experiment.
Materials and methods. The study involved 60 Wistar laboratory rats divided into 2 groups (experimental, which was under the combined influence of general vibration and noise (at the level of 1.5 MPU), and comparison group (intact animals)) There were 30 individuals (15 females and 15 males) each, in which a morphohistological analysis of the tissues of internal organs was carried out in 180-day dynamics with an interval of 2 months (after 60-120-180 days). In addition, for background analysis, five individuals of each sex were selected for the comparison group. On the 0th day (background), and after 60-120-180 days, 10 rats from each group were euthanized using chloroform (3-5-10 ml of chloroform was injected into an exicator using a syringe until the rats were in a state of narcosis) and necropsied for histopathological studies. The sections were stained with hematoxylin and eosin.
Animal experiments were conducted in accordance with the European Convention for the Protection of Animals Used for Scientific Purposes, after approval by the Ethics Committee of the Novosibirsk Research Institute of Hygiene.
Results and their discussion. The results of the study indicate the negative combined effect of whole-body vibration and noise (1.5 MAC) on the morphological structure of the internal organs of Wistar rats. Primary pathological changes are already detected after 60 days of the experiment, while after 180 days there is a significant intensification of structural disturbances, especially in the heart, liver, and kidneys.
Conclusion. The development of structural pathological changes in two or more internal organs in rats of the experimental group (exposed to combined effects of general vibration and noise) can be considered as one of the indicators of premature aging in warm-blooded organisms when exposed to physical factors of occupational hazards for a long time.
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Cheng T, Zhang B, Guo J, Pikhart H. Association between work stress and health behaviours in Korean and Japanese ageing studies: a cross-sectional study. BMJ Open. 2022; 12(8): e063538. doi: 10.1136/bmjopen-2022-063538
Karimov DD, Erdman VV, Kudoyarov ER, Valova YaV, Smolyankin DA, Repina EF, Karimov DO. Influence of occupational risk factors on human aging (literature review). Hygiene and Sanitation. 2022; 101(4): 375-381. Russian (Каримов Д.Д., Эрдман В.В., Кудояров Э.Р., Валова Я.В., Смолянкин Д.А., Репина Э.Ф., Каримов Д.О. Профессиональные факторы риска и старение человека (обзор литературы) //Гигиена и санитария. 2022. Т. 101, № 4. С. 375-381.) doi: 10.47470/0016-9900-2022-101-4-375-381
Harrison J, Dawson L. Occupational health: Meeting the challenges of the next 20 Years. Saf Health Work. 2016; 7(2): 143-149. doi: 10.1016/j.shaw.2015.12.004
Almukhametov AA, Petrov IV, Petrova FS, Rybakova SV, Amirova TK. Epidemiology of occupational diseases in the Russian Federation сharacteristics causes and risks. J Res Med Dent Sci. 2020; 8(7): 465-470
Lee J, Kim J. Physical agents and occupational disease compensation: noise, vibration, radiation, and other physical agents. J Korean Med Sci. 2014; 29: 72-77. doi: 10.3346/jkms.2014.29.S.S72
Long working hours increasing deaths from heart disease and stroke: WHO, 2021. https://www.who.int/news/item/17-05-2021-long-working-hours-increasing-deaths-from-heart-disease-and-stroke-who-ilo
Sadrtdinova GR, Masyagutova LM, Chudnovets GM, Gazizova NR. The state of enzymatic liver function in workers in the presence of chromium in the air of the work area. Occupational medicine and human ecology. 2019; (2): 57-62. Russian (Садртдинова Г.Р., Масягутова Л.М., Чудновец Г.М., Газизова Н.Р. Состояние ферментативной функции печени у работников при наличии хрома в воздухе рабочей зоны //Медицина труда и экология человека. 2019. № 2. С. 57-62.) doi:10.24411/2411-3794-2019-10022
Sheenkova MV, Rushkevich OP, Yatsyna IV. Features of metabolic pathology of the liver under the influence of industrial aerosols. Hygiene and sanitation. 2021; 100(9): 943-946. Russian (Шеенкова М.В., Рушкевич О.П., Яцына И.В. Особенности метаболической патологии печени в условиях воздействия промышленных аэрозолей //Гигиена и санитария. 2021. Т. 100, № 9. С. 943-946.) doi: 10.47470/0016-9900-2021-100-9-943-946
Zhang H, Li H, Peng Z, Cao J, Bao J, Li L, et al. Meta-analysis of the effect of low-level occupational benzene exposure on human peripheral blood leukocyte counts in China. J Environ Sci (China). 2022; 114: 204-210. doi: 10.1016/j.jes.2021.08.035
Sheppard A, Ralli M, Gilardi A, Salvi R. Occupational Noise: Auditory and Non-Auditory Consequences. Int J Environ Res Public Health. 2020; 17 (23): 8963. doi:10.3390/ijerph17238963
Baudin C, LefÈvre M, Champelovier P, Lambert J, Laumon B, Evrard AS. Self-rated health status in relation to aircraft noise exposure, noise annoyance or noise sensitivity: the results of a cross-sectional study in France. BMC Public Health. 2021; 21(1): 116. doi: 10.1186/s12889-020-10138-0
Vorobieva VV, Shabanov PD. Exposure to whole body vibration impairs the functional activity of the energy producing system in rabbit myocardium. Biophysics. 2019; 64: 251-255. Russian (Воробьева В.В., Шабанов П.Д. Воздействие вибрации всего тела нарушает функциональную активность энергопродуцирующей системы в миокарде кролика //Биофизика. 2019. № 64. 251-255.) doi: 10.1134/S0006350919020210
Ivanov AV, Tremasov MYa, Akhmetov FG, Papunidi EK, Semyonov E.I. Combined Effects of physical and chemical factors on clinical and biochemical parameters in animals. Veterinarian. 2007; 4: 4-5. Russian (Иванов А.В. Тремасов М.Я., Ахметов Ф.Г., Папуниди Э.К., Семенов Э.И. Сочетанное воздействие физических и химических факторов на клинические и биохимические показатели животных //Ветеринарный врач. 2007. № 4. С. 4-5)
Beyrami S, Ramezanifar S, Golmohammadi H, Nasiri R, Mahmoudi S, Ramezanifar E. Changes in Oxidative Stress Parameters in Terms of Simultaneous Exposure to Physical and Chemical Factors: A Systematic Review. Iran J Public Health. 2023; 52(6): 1129-1139.) doi: 10.18502/ijph.v52i6.12955
Savchenko OA, Chuenko NF, Plotnikova OV, Savchenko OA, Savchenko OO. Factors and biomarkers associated with accelerated aging. National Priorities of Russia. 2024; 3(54): 45-52. Russian (Савченко О.А., Чуенко Н.Ф., Плотникова О.В., Савченко О.А., Савченко О.О. Факторы и биомаркеры, связанные с ускоренным старением //Национальные приоритеты России. 2024. Т. 3, № 54. С. 45-52)
Peschnikova EA. Review of the main pathophysiological mechanisms of aging. Young scientist. 2023; 23(470): 179-182. Russian (Песчанникова Е.А. Обзор основных патофизиологических механизмов старения организма //Молодой ученый. 2023. № 23(470). С. 179-182.) URL: https://moluch.ru/archive/470/102778